Hotspot

rps2

LSC · 16658-17368 · 711 bp

Back to Reevesia

Pi 0.00148

MarkerSeek score 28.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCTCTTTTGTGCGATTCAGGA	AGAGAGAGGAAGTGTGAAGAGA	42.9	45.5	57.6	57.6	818 bp	1.0	50.0
rps2_p2	TCTCTTTTGTGCGATTCAGGA	GAGAGAGGAAGTGTGAAGAGA	42.9	47.6	57.6	56.1	817 bp	1.0	46.1
rps2_p3	TCTCTTTTGTGCGATTCAGGA	AGAGAGAGGAAGTGTGAAGAG	42.9	47.6	57.6	56.1	818 bp	1.0	46.1
rps2_p4	CTCTTTTGTGCGATTCAGGA	AGAGAGAGGAAGTGTGAAGAGA	45.0	45.5	56.1	57.6	817 bp	1.0	45.8
rps2_p5	TCTCTTTTGTGCGATTCAGG	AGAGAGAGGAAGTGTGAAGAGA	45.0	45.5	56.1	57.6	818 bp	1.0	45.8