Hotspot

rpl2

IRa · 160262-161780 · 1519 bp

Back to Reevesia

Pi 0.000366

MarkerSeek score 27.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1519
variable_sites: 1
pi: 0.000366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1611 bp	1.0	40.0
rpl2_p2	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1618 bp	1.0	40.0
rpl2_p3	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCT	50.0	30.4	58.3	54.3	1611 bp	1.0	40.0
rpl2_p4	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCT	50.0	30.4	58.3	54.3	1618 bp	1.0	40.0
rpl2_p5	ACGCTTCAACCGGGTTATTC	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.6	55.4	1612 bp	1.0	40.0