Hotspot

ndhA

SSC · 126576-128774 · 2199 bp

Back to Reevesia

Pi 0.00131

MarkerSeek score 50.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2199
variable_sites: 8
pi: 0.00131
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	CCCGCCGGGTTTTATCAATT	47.6	50.0	58.1	58.9	2417-2424 bp	1.0	64.7
ndhA_p2	ACCCAGTTACCATAGGGAACA	CCGCCGGGTTTTATCAATTT	47.6	45.0	58.1	57.0	2416-2423 bp	1.0	64.2
ndhA_p3	TGAACCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	47.6	50.0	57.8	58.9	2420-2427 bp	1.0	63.6
ndhA_p4	ACCCAGTTACCATAGGGAACA	GCCGGGTTTTATCAATTTGCA	47.6	42.9	58.1	58.0	2414-2421 bp	1.0	63.5
ndhA_p5	TGAACCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	47.6	45.0	57.8	57.0	2419-2426 bp	1.0	63.1