Hotspot

clpP

LSC · 76011-78174 · 2164 bp

Back to Reevesia

Pi 0.00435
MarkerSeek score 57.00
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2164
variable_sites
20
pi
0.00435
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TGTGGATTGGTGTTAAATCGTTG ACGCTTCCACATCAAAGTGA 39.1 45.0 57.9 57.7 2235-2261 bp 1.0 54.2
clpP_p2 TGTGGATTGGTGTTAAATCGT ACGCTTCCACATCAAAGTGA 38.1 45.0 55.8 57.7 2235-2261 bp 1.0 53.5
clpP_p3 TGTGGATTGGTGTTAAATCGTT ACGCTTCCACATCAAAGTGA 36.4 45.0 56.5 57.7 2235-2261 bp 1.0 51.5
clpP_p4 TTGTGGATTGGTGTTAAATCGT ACGCTTCCACATCAAAGTGA 36.4 45.0 56.5 57.7 2236-2262 bp 1.0 51.5
clpP_p5 GTGGATTGGTGTTAAATCGTTG ACGCTTCCACATCAAAGTGA 40.9 45.0 56.3 57.7 2234-2260 bp 1.0 49.2