Hotspot

ccsA-ndhD

SSC · 122051-122366 · 316 bp

Back to Reevesia

Pi 0.02

MarkerSeek score 47.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 316
variable_sites: 13
pi: 0.02
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAATTCCGCACTTGTGGC	ATCCGGATTTCGTCCTCTCA	50.0	50.0	58.0	58.2	507-515 bp	1.0	80.1
ccsA-ndhD_p2	GTAAATTCCGCACTTGTGGC	TCCGGATTTCGTCCTCTCAT	50.0	50.0	58.0	58.2	506-514 bp	1.0	80.1
ccsA-ndhD_p3	TAAATTCCGCACTTGTGGCT	ATCCGGATTTCGTCCTCTCA	45.0	50.0	58.1	58.2	506-514 bp	1.0	79.5
ccsA-ndhD_p4	TAAATTCCGCACTTGTGGCT	TCCGGATTTCGTCCTCTCAT	45.0	50.0	58.1	58.2	505-513 bp	1.0	79.5
ccsA-ndhD_p5	GTAAATTCCGCACTTGTGGC	GGTGAGAAGGTCGAAACGAT	50.0	50.0	58.0	57.6	481-489 bp	1.0	79.0