Hotspot

psbE-petL

LSC · 70650-71555 · 906 bp

Back to Reevesia

Pi 0.00264

MarkerSeek score 54.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 5
pi: 0.00264
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	950-995 bp	1.0	60.8
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	TGAGGTTATAGTTAAAGCCGCT	55.0	40.9	58.2	57.3	945-990 bp	1.0	60.5
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.4	57.3	943-988 bp	1.0	59.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTATAGTTAAAGCCGCTAGC	50.0	45.5	57.8	57.0	947-992 bp	1.0	58.9
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	GGTTATAGTTAAAGCCGCTAGC	55.0	45.5	58.2	57.0	942-987 bp	1.0	58.6