Hotspot

rps4-trnT-UGU

LSC · 49334-49899 · 566 bp

Back to Reevesia

Pi 0.01

MarkerSeek score 61.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 15
pi: 0.01
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	AGGTTAGAGCCTCGCATTTG	50.0	50.0	58.0	58.0	674-748 bp	1.0	85.3
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	AGGTTAGAGCCTCGCATTTG	50.0	50.0	58.1	58.0	678-752 bp	1.0	84.9
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	GAGCCTCGCATTTGTAATGC	50.0	50.0	58.0	57.9	668-742 bp	1.0	84.6
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	GAGCCTCGCATTTGTAATGC	50.0	50.0	58.1	57.9	672-746 bp	1.0	84.3
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	647-721 bp	1.0	83.7