Hotspot

petN-psbM

LSC · 30141-31297 · 1157 bp

Back to Reevesia

Pi 0.00643

MarkerSeek score 51.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 16
pi: 0.00643
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ATTGCTACAGCGCTGTTCAT	45.0	45.0	58.0	58.3	1301-1314 bp	1.0	79.9
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TATTGCTACAGCGCTGTTCA	45.0	45.0	58.0	57.6	1302-1315 bp	1.0	78.9
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	ATTGCTACAGCGCTGTTCAT	50.0	45.0	57.8	58.3	1307-1320 bp	1.0	78.4
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TTGCTACAGCGCTGTTCATT	45.0	45.0	58.0	58.5	1300-1313 bp	1.0	78.1
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TATTGCTACAGCGCTGTTCA	50.0	45.0	57.8	57.6	1308-1321 bp	1.0	77.4