Hotspot

atpF-atpH

LSC · 13619-14216 · 598 bp

Back to Reevesia

Pi 0.00427

MarkerSeek score 53.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 6
pi: 0.00427
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	682-685 bp	1.0	80.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.9	58.0	710-713 bp	1.0	80.1
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	50.0	50.0	58.0	58.5	683-686 bp	1.0	77.2
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	50.0	50.0	58.0	58.5	688-691 bp	1.0	76.7
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	50.0	50.0	57.9	58.5	711-714 bp	1.0	76.5