Hotspot

accD-psaI

LSC · 60226-60861 · 636 bp

Back to Reaumuria

Pi 0.0411

MarkerSeek score 72.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 25
pi: 0.0411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAACCCAACGCTTACATTGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	878-898 bp	1.0	84.9
accD-psaI_p2	TACTACTGGTGGGGTGACAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	933-953 bp	1.0	84.3
accD-psaI_p3	CTACTACTGGTGGGGTGACA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	934-954 bp	1.0	84.2
accD-psaI_p4	CCGGGTACTTGGGATCCTAT	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1343-1363 bp	1.0	83.4
accD-psaI_p5	GAACCCAACGCTTACATTGC	CCCCACTGTGCCTAAACAAT	50.0	50.0	58.0	58.1	950 bp	0.5	59.2