Hotspot

rps4-trnT-UGU

LSC · 47632-48172 · 541 bp

Back to Reaumuria

Pi 0.0301

MarkerSeek score 69.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 15
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	ACTTCCACCTCTAGGCCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	652-694 bp	1.0	84.4
rps4-trnT-UGU_p2	ACTTCCACCTCTAGGCCTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	620-662 bp	1.0	83.6
rps4-trnT-UGU_p3	ACTTCCACCTCTAGGCCTTT	ATCAGCCTGCTTAGCTCAGA	50.0	50.0	58.0	58.5	666-708 bp	1.0	81.3
rps4-trnT-UGU_p4	ACTTCCACCTCTAGGCCTTT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.0	57.5	650-692 bp	1.0	81.2
rps4-trnT-UGU_p5	ACTTCCACCTCTAGGCCTTT	TCAGAGGTTAGAGCATCGCA	50.0	50.0	58.0	58.5	651-693 bp	1.0	81.2