Hotspot

trnD-GUC-trnY-GUA

LSC · 31289-31681 · 393 bp

Back to Reaumuria

Pi 0.0308
MarkerSeek score 68.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
393
variable_sites
12
pi
0.0308
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA ATTGACTCTTCCTGGGTCGA 50.0 50.0 58.5 58.1 517-520 bp 1.0 78.2
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA TTGACTCTTCCTGGGTCGAT 50.0 50.0 58.5 58.1 516-519 bp 1.0 78.2
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GCAAGATGTCTACGCTGGTT 50.0 50.0 58.5 58.3 458-461 bp 1.0 76.8
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GTTGGCAAGATGTCTACGCT 50.0 50.0 58.5 58.3 462-465 bp 1.0 76.8
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA TTGGCAAGATGTCTACGCTG 50.0 50.0 58.5 58.3 461-464 bp 1.0 76.8