Hotspot

psbM-trnD-GUC

LSC · 30193-31214 · 1022 bp

Back to Reaumuria

Pi 0.0327

MarkerSeek score 70.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 33
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1132-1133 bp	1.0	57.0
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	1135-1136 bp	1.0	55.5
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	1139-1140 bp	1.0	54.8
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	1139-1140 bp	1.0	54.1
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	1138-1139 bp	1.0	54.1