Hotspot

trnS-GCU-trnG-UCC

LSC · 8351-9258 · 908 bp

Back to Reaumuria

Pi 0.0295

MarkerSeek score 73.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 25
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACAAATGACTCGTACACCGG	AATCAAACCGAAAGACCCCT	50.0	45.0	57.9	57.0	1027-1044 bp	1.0	77.6
trnS-GCU-trnG-UCC_p2	ACAAATGACTCGTACACCGG	ATCAAACCGAAAGACCCCTT	50.0	45.0	57.9	57.0	1026-1043 bp	1.0	77.6
trnS-GCU-trnG-UCC_p3	CCCTCGGTACAAATGACTCG	AATCAAACCGAAAGACCCCT	55.0	45.0	58.1	57.0	1035-1052 bp	1.0	77.5
trnS-GCU-trnG-UCC_p4	CCCTCGGTACAAATGACTCG	ATCAAACCGAAAGACCCCTT	55.0	45.0	58.1	57.0	1034-1051 bp	1.0	77.5
trnS-GCU-trnG-UCC_p5	GACTCGTACACCGGATTAGC	ATCAAACCGAAAGACCCCTT	55.0	45.0	57.9	57.0	1020-1037 bp	1.0	77.2