Hotspot

psbA-trnK-UUU

LSC · 1357-1594 · 238 bp

Back to Reaumuria

Pi 0.0588

MarkerSeek score 71.60

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 14
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TACAGAAGCGACCCCATAGG	ACTCCATCCGACTAGTTCCG	55.0	55.0	58.6	58.6	335 bp	1.0	73.7
psbA-trnK-UUU_p2	ATCCAGTTACAGAAGCGACC	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.3	58.6	342 bp	1.0	73.0
psbA-trnK-UUU_p3	AGTTACAGAAGCGACCCCAT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.7	58.6	338 bp	1.0	72.7
psbA-trnK-UUU_p4	TACAGAAGCGACCCCATAGG	ACTAGTTCCGGGTTCGAGTC	55.0	55.0	58.6	58.8	325 bp	1.0	72.1
psbA-trnK-UUU_p5	TACAGAAGCGACCCCATAGG	GACTAGTTCCGGGTTCGAGT	55.0	55.0	58.6	58.8	326 bp	1.0	72.1