Hotspot

ndhF

SSC · 111144-113369 · 2226 bp

Back to Rauvolfia

Pi 0.0114

MarkerSeek score 51.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 38
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	ACTCGGGAAAAGAGGTAGGT	50.0	50.0	58.0	57.7	2936-2973 bp	1.0	80.3
ndhF_p2	ACAGGAACAAGAGGGATCCA	ACTCGGGAAAAGAGGTAGGT	50.0	50.0	58.0	57.7	2812-2849 bp	1.0	80.3
ndhF_p3	AAGAGGGATCCACCGAAGAA	ACTCGGGAAAAGAGGTAGGT	50.0	50.0	58.0	57.7	2804-2841 bp	1.0	80.3
ndhF_p4	AGGGTGGAAAGTGAGGAAGA	ACTCGGGAAAAGAGGTAGGT	50.0	50.0	57.9	57.7	2877-2914 bp	1.0	79.7
ndhF_p5	AGGGATCCACCGAAGAAGAT	ACTCGGGAAAAGAGGTAGGT	50.0	50.0	57.8	57.7	2801-2838 bp	1.0	79.2