Hotspot

ndhG-ndhI

SSC · 119731-120065 · 335 bp

Back to Rauvolfia

Pi 0.0139

MarkerSeek score 61.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 335
variable_sites: 7
pi: 0.0139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATCAGACCCGATCCCAGAAA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	58.1	58.3	485-493 bp	1.0	79.4
ndhG-ndhI_p2	ATCAGACCCGATCCCAGAAA	CAAATTGCTTTGGGTCGGTT	50.0	45.0	58.1	57.5	484-492 bp	1.0	77.6
ndhG-ndhI_p3	ATCAGACCCGATCCCAGAAA	GTCGGTTACCAACGTCAGTA	50.0	50.0	58.1	57.3	471-479 bp	1.0	76.3
ndhG-ndhI_p4	ATCAGACCCGATCCCAGAAA	CGGTTACCAACGTCAGTAGT	50.0	50.0	58.1	57.3	469-477 bp	1.0	76.2
ndhG-ndhI_p5	ACCCGATCCCAGAAAGACTA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.5	58.3	480-488 bp	1.0	76.0