Hotspot

ndhF-rpl32

SSC · 113370-114172 · 803 bp

Back to Rauvolfia

Pi 0.0203

MarkerSeek score 66.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 803
variable_sites: 24
pi: 0.0203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTATGAGCCACCCGTATTG	TCCAAATATTTTTCCGAATACGCT	55.0	33.3	58.1	57.3	864-899 bp	1.0	47.7
ndhF-rpl32_p2	GGAACGGGAAGTGGAATGAA	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	57.8	57.3	885-920 bp	1.0	47.1
ndhF-rpl32_p3	AAGGTATGAGCCACCCGTAT	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	58.2	57.3	866-901 bp	1.0	47.1
ndhF-rpl32_p4	AGGTATGAGCCACCCGTATT	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	58.2	57.3	865-900 bp	1.0	47.1
ndhF-rpl32_p5	CGGGAAGTGGAATGAAAGGT	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	57.8	57.3	881-916 bp	1.0	47.0