Hotspot

rps19-rpl2

IRb · 85437-85508 · 72 bp

Back to Rauvolfia

Pi 0.0278

MarkerSeek score 65.80

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 72
variable_sites: 3
pi: 0.0278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTTGGCTACAAAAGGATT	TTGATTCTTCGTCGCCGTAG	40.0	50.0	55.1	58.1	131-135 bp	1.0	59.8
rps19-rpl2_p2	AATGGTTGGCTACAAAAGGA	TTGATTCTTCGTCGCCGTAG	40.0	50.0	55.1	58.1	133-137 bp	1.0	59.7
rps19-rpl2_p3	AATGGTTGGCTACAAAAGGA	CGAGCCCCAATTGGTAGAAA	40.0	50.0	55.1	57.9	226-230 bp	1.0	58.1
rps19-rpl2_p4	TGGTTGGCTACAAAAGGATT	CGAGCCCCAATTGGTAGAAA	40.0	50.0	55.1	57.9	224-228 bp	1.0	58.1
rps19-rpl2_p5	TGGTTGGCTACAAAAGGATT	CACAACCCCTTGGGGTTATC	40.0	55.0	55.1	58.2	198-202 bp	1.0	58.1