Hotspot

clpP-psbB

LSC · 73462-73929 · 468 bp

Back to Rauvolfia

Pi 0.015

MarkerSeek score 70.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 9
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGTCAACCCAAGATGCATCT	GGCCGGGATCATTCAATACA	50.0	50.0	58.3	57.7	577-587 bp	1.0	81.0
clpP-psbB_p2	TCCAGGACTTCGGAAAGGTA	GGCCGGGATCATTCAATACA	50.0	50.0	57.7	57.7	552-562 bp	1.0	80.6
clpP-psbB_p3	CCAGGACTTCGGAAAGGTAC	GGCCGGGATCATTCAATACA	55.0	50.0	57.6	57.7	551-561 bp	1.0	80.1
clpP-psbB_p4	ACGTCAACCCAAGATGCATC	GGCCGGGATCATTCAATACA	50.0	50.0	58.5	57.7	578-588 bp	1.0	78.7
clpP-psbB_p5	GATGCATCTTCCTCTCCAGG	GGCCGGGATCATTCAATACA	55.0	50.0	57.5	57.7	566-576 bp	1.0	78.7