Hotspot

trnL-UAA-trnF-GAA

LSC · 48592-48948 · 357 bp

Back to Rauvolfia

Pi 0.0336

MarkerSeek score 54.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 18
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AGACTCAACGGAAGCTGTTC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	773-786 bp	1.0	83.3
trnL-UAA-trnF-GAA_p2	GACTCAACGGAAGCTGTTCT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	772-785 bp	1.0	83.3
trnL-UAA-trnF-GAA_p3	GAGACTCAACGGAAGCTGTT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	774-787 bp	1.0	83.3
trnL-UAA-trnF-GAA_p4	CTTCTTCGAGCTCGGAATCC	TCTACCAGCTGAGCTATCCC	55.0	55.0	58.2	58.0	1057-1084 bp	1.0	83.0
trnL-UAA-trnF-GAA_p5	CTTCTTCGAGCTCGGAATCC	ACTTTTTGAACGGGAGGGAC	55.0	50.0	58.2	58.0	1317-1344 bp	1.0	82.6