Hotspot

trnC-GCA-petN

LSC · 27786-28579 · 794 bp

Back to Rauvolfia

Pi 0.0122

MarkerSeek score 62.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 14
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	720-914 bp	1.0	84.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	718-912 bp	1.0	84.6
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	721-915 bp	1.0	84.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	666-860 bp	1.0	83.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTCTTCCCCACACTACGAGT	50.0	50.0	58.4	58.0	736-930 bp	1.0	82.4