Hotspot

atpI-rps2

LSC · 14938-15164 · 227 bp

Back to Rauvolfia

Pi 0.0147

MarkerSeek score 61.90

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 5
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GAAATGCTGGCCTACTTCCA	TGCAATTTGTGAGGGTCGTT	50.0	45.0	57.9	58.3	345 bp	1.0	77.6
atpI-rps2_p2	GAAATGCTGGCCTACTTCCA	TTGCAATTTGTGAGGGTCGT	50.0	45.0	57.9	58.3	346 bp	1.0	77.6
atpI-rps2_p3	GAAATGCTGGCCTACTTCCA	GCAATTTGTGAGGGTCGTTC	50.0	50.0	57.9	57.7	344 bp	1.0	77.5
atpI-rps2_p4	AAATGCTGGCCTACTTCCAC	TGCAATTTGTGAGGGTCGTT	50.0	45.0	58.2	58.3	344 bp	1.0	77.4
atpI-rps2_p5	AAATGCTGGCCTACTTCCAC	TTGCAATTTGTGAGGGTCGT	50.0	45.0	58.2	58.3	345 bp	1.0	77.4