Hotspot

rpl32-trnL-TAG

SSC · 116136-116959 · 824 bp

Back to Raukaua

Pi 0.00775

MarkerSeek score 59.40

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 824
variable_sites: 12
pi: 0.00775
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	335-951 bp	1.0	82.3
rpl32-trnL-TAG_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	334-952 bp	1.0	82.3
rpl32-trnL-TAG_p3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	334-961 bp	1.0	81.4
rpl32-trnL-TAG_p4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	324-940 bp	1.0	81.2
rpl32-trnL-TAG_p5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	323-941 bp	1.0	81.2