Hotspot

rps18-rpl20

LSC · 70700-70963 · 264 bp

Back to Raukaua

Pi 0.00827

MarkerSeek score 55.60

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 264
variable_sites: 4
pi: 0.00827
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GAACTGCTGGTCTTAGAGCC	GAAAGAATCCACCGGAATAA	55.0	40.0	58.0	53.0	321-322 bp	1.0	41.0
rps18-rpl20_p2	TTGAAAGAACCGAGTCGACC	GAAAGAATCCACCGGAATAA	50.0	40.0	57.9	53.0	345-346 bp	1.0	40.9
rps18-rpl20_p3	GAACTGCTGGTCTTAGAGCC	GCTTCTGAATCGTAAAATACTTGC	55.0	37.5	58.0	57.0	414-415 bp	1.0	40.8
rps18-rpl20_p4	GAACTGCTGGTCTTAGAGCC	TCTGAATCGTAAAATACTTGCACA	55.0	33.3	58.0	57.0	411-412 bp	1.0	40.8
rps18-rpl20_p5	TTGAAAGAACCGAGTCGACC	GCTTCTGAATCGTAAAATACTTGC	50.0	37.5	57.9	57.0	438-439 bp	1.0	40.7