Hotspot

trnC-GCA-petN

LSC · 29518-30207 · 690 bp

Back to Raukaua

Pi 0.00749
MarkerSeek score 58.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
690
variable_sites
9
pi
0.00749
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CGATATGGCCGAGTGGTAAG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 57.9 58.0 791-807 bp 1.0 81.0
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 751-767 bp 1.0 81.0
trnC-GCA-petN_p3 CGATATGGCCGAGTGGTAAG CAGCCCAAGCAAGACTTACT 55.0 50.0 57.9 57.8 785-801 bp 1.0 79.6
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 745-761 bp 1.0 79.5
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 769-785 bp 1.0 78.8