Hotspot

rpoB-trnC-GCA

LSC · 28142-29446 · 1305 bp

Back to Raukaua

Pi 0.00436

MarkerSeek score 55.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1305
variable_sites: 11
pi: 0.00436
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1319-1351 bp	1.0	80.4
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1359-1391 bp	1.0	80.2
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCTCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1341-1373 bp	1.0	78.0
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	CCTTACCACTCGGCCATATC	50.0	55.0	57.8	57.5	1320-1352 bp	1.0	77.3
rpoB-trnC-GCA_p5	TCCCTCATTTCCATCTCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	1378-1410 bp	1.0	74.7