Hotspot

trnH-GTG-psbA

LSC · 76-484 · 409 bp

Back to Raukaua

Pi 0.0289

MarkerSeek score 64.40

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 21
pi: 0.0289
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	1066-1102 bp	1.0	82.9
trnH-GTG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	1072-1108 bp	1.0	82.9
trnH-GTG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	1067-1103 bp	1.0	82.9
trnH-GTG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	1068-1104 bp	1.0	82.9
trnH-GTG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	1080-1116 bp	1.0	82.8