Hotspot

rpoC2

LSC · 15551-19693 · 4143 bp

Back to Raphanus

Pi 0.000805

MarkerSeek score 27.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4143
variable_sites: 5
pi: 0.000805
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AATTTGTTAACATGCCGCCG	GATTGGTGCATTGTTCACGG	45.0	50.0	58.0	58.0	682 bp	1.0	80.4
rpoC2_p2	AATTTGTTAACATGCCGCCG	GGATTGGTGCATTGTTCACG	45.0	50.0	58.0	58.0	683 bp	1.0	80.4
rpoC2_p3	AATTTGTTAACATGCCGCCG	CAGGTGTTAGAAGTCCGCTC	45.0	55.0	58.0	58.0	1223 bp	1.0	80.3
rpoC2_p4	ACGAGCCGAGCCATTATTTT	CAGGTGTTAGAAGTCCGCTC	45.0	55.0	58.0	58.0	820 bp	1.0	80.2
rpoC2_p5	AACATAATGACACCGAGCCC	CAGGTGTTAGAAGTCCGCTC	50.0	55.0	58.0	58.0	1189 bp	1.0	80.1