Hotspot

psbA

LSC · 313-1374 · 1062 bp

Back to Raphanus

Pi 0.00126

MarkerSeek score 36.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GAAGGCTTATATTGCTCGTT	ATTTGTGTGCTTGGGAGTCC	40.0	50.0	53.5	58.4	1173 bp	1.0	41.0
psbA_p2	GAAGGCTTATATTGCTCGTTT	ATTTGTGTGCTTGGGAGTCC	38.1	50.0	54.4	58.4	1173 bp	1.0	40.1
psbA_p3	GAAGGCTTATATTGCTCGTTTT	ATTTGTGTGCTTGGGAGTCC	36.4	50.0	55.2	58.4	1173 bp	1.0	40.1
psbA_p4	AAGGCTTATATTGCTCGTTT	ATTTGTGTGCTTGGGAGTCC	35.0	50.0	53.0	58.4	1172 bp	1.0	40.1
psbA_p5	AGGCTTATATTGCTCGTTTT	ATTTGTGTGCTTGGGAGTCC	35.0	50.0	53.0	58.4	1171 bp	1.0	40.1