Hotspot

atpI

LSC · 13646-14395 · 750 bp

Back to Raphanus

Pi 0.00178

MarkerSeek score 47.40

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 750
variable_sites: 2
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	ACCAACTATTCGATAATTGGGCT	TCTTATTTCTGTCAGAGGGCA	39.1	42.9	58.0	56.4	820-821 bp	1.0	40.3
atpI_p2	ACCAACTATTCGATAATTGGGCT	TCTTATTTCTGTCAGAGGGCAA	39.1	40.9	58.0	57.1	820-821 bp	1.0	40.3
atpI_p3	ACCAACTATTCGATAATTGGGCT	TTCTTATTTCTGTCAGAGGGCA	39.1	40.9	58.0	57.1	821-822 bp	1.0	40.3
atpI_p4	ACCAACTATTCGATAATTGGGC	TCTTATTTCTGTCAGAGGGCA	40.9	42.9	56.6	56.4	820-821 bp	1.0	40.3
atpI_p5	ACCAACTATTCGATAATTGGGCT	AGTTCTTATTTCTGTCAGAGGGC	39.1	43.5	58.0	58.2	823-824 bp	1.0	40.3