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rps15-ycf1

SSC · 122506-122877 · 372 bp

Back to Raphanus

Pi 0.00717

MarkerSeek score 46.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 372
variable_sites: 4
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGATCCCTTGCTTTCT	TGTATACGCGATTTCAAATGCA	42.9	36.4	57.2	57.1	460 bp	1.0	43.4
rps15-ycf1_p2	TCAACAGATCCCTTGCTTTCT	TGGCCCAATTTTCGATTAGAAG	42.9	40.9	57.2	57.0	554 bp	1.0	42.4
rps15-ycf1_p3	TCAACAGATCCCTTGCTTTCT	TGGCCCAATTTTCGATTAGA	42.9	40.0	57.2	55.0	554 bp	1.0	42.3
rps15-ycf1_p4	TCAACAGATCCCTTGCTTTCT	GCTTGTATGAATCGCTATTGGT	42.9	40.9	57.2	56.9	527 bp	1.0	41.6
rps15-ycf1_p5	ACAGATCCCTTGCTTTCTTCT	TGTATACGCGATTTCAAATGCA	42.9	36.4	56.9	57.1	457 bp	1.0	41.1