Hotspot

psbH-petB

LSC · 73728-73862 · 135 bp

Back to Raphanus

Pi 0.0198

MarkerSeek score 52.60

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 4
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTGTTGCAATGGCGCTATTT	ACAGACCCCCTTCTCTGTAC	45.0	55.0	58.0	58.1	284 bp	1.0	80.6
psbH-petB_p2	TGTTGCAATGGCGCTATTTG	ACAGACCCCCTTCTCTGTAC	45.0	55.0	58.0	58.1	283 bp	1.0	80.6
psbH-petB_p3	GTGTTGCAATGGCGCTATTT	GATGACAGACCCCCTTCTCT	45.0	55.0	58.0	58.1	288 bp	1.0	80.1
psbH-petB_p4	GTGTTGCAATGGCGCTATTT	AGATGACAGACCCCCTTCTC	45.0	55.0	58.0	58.1	289 bp	1.0	80.0
psbH-petB_p5	TGTTGCAATGGCGCTATTTG	GATGACAGACCCCCTTCTCT	45.0	55.0	58.0	58.1	287 bp	1.0	80.0