Hotspot

rps12-clpP

LSC · 68599-68779 · 181 bp

Back to Raphanus

Pi 0.0295

MarkerSeek score 58.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 8
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	294 bp	1.0	79.7
rps12-clpP_p2	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	346 bp	1.0	79.4
rps12-clpP_p3	CCGAGTACATGTTCCTCGTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.8	57.9	347 bp	1.0	79.4
rps12-clpP_p4	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	289 bp	1.0	79.1
rps12-clpP_p5	CACCCGAGTACATGTTCCTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.7	57.9	350 bp	1.0	78.6