Hotspot

rpl20-rps12

LSC · 67723-68484 · 762 bp

Back to Raphanus

Pi 0.00175

MarkerSeek score 41.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	875 bp	1.0	78.3
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	889 bp	1.0	78.0
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	880 bp	1.0	77.7
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	819 bp	1.0	77.6
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	894 bp	1.0	77.4