Hotspot

psaJ-rpl33

LSC · 65924-66348 · 425 bp

Back to Raphanus

Pi 0.00471

MarkerSeek score 45.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 3
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTCGTTTCGTTAGCAGGT	TGGAAATTCCAGCCGATTCT	50.0	45.0	58.5	57.2	595-596 bp	1.0	70.1
psaJ-rpl33_p2	TGGTTCGTTTCGTTAGCAGG	TGGAAATTCCAGCCGATTCT	50.0	45.0	58.5	57.2	596-597 bp	1.0	70.1
psaJ-rpl33_p3	GGTTCGTTTCGTTAGCAGGT	TGGAAATTCCAGCCGATTCTT	50.0	42.9	58.5	57.9	595-596 bp	1.0	67.9
psaJ-rpl33_p4	TGGTTCGTTTCGTTAGCAGG	TGGAAATTCCAGCCGATTCTT	50.0	42.9	58.5	57.9	596-597 bp	1.0	67.9
psaJ-rpl33_p5	CCGTAGCACCGGTACTAAGT	TGGAAATTCCAGCCGATTCT	55.0	45.0	58.9	57.2	622-623 bp	1.0	66.8