Hotspot

ndhC-trnV-UAC

LSC · 49418-50093 · 676 bp

Back to Raphanus

Pi 0.00592
MarkerSeek score 50.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
676
variable_sites
6
pi
0.00592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCCTTCGTAATTGGAGAT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.4 57.9 810 bp 1.0 75.0
ndhC-trnV-UAC_p2 GCCCCTTCGTAATTGGAGAT CAGGTCTACGGTTCGAATCC 50.0 55.0 57.4 57.8 819 bp 1.0 73.9
ndhC-trnV-UAC_p3 GCCCCTTCGTAATTGGAGAT CGGTTCGAATCCGTATAGCC 50.0 55.0 57.4 58.3 811 bp 1.0 73.1
ndhC-trnV-UAC_p4 GCCCCTTCGTAATTGGAGAT GCAGGTCTACGGTTCGAATC 50.0 55.0 57.4 58.4 820 bp 1.0 72.0
ndhC-trnV-UAC_p5 GGCCCCTTCGTAATTGGAG GGTTCGAATCCGTATAGCCC 57.9 55.0 57.9 57.9 811 bp 1.0 71.3