Hotspot

trnT-UGU-trnL-UAA

LSC · 45507-46321 · 815 bp

Back to Raphanus

Pi 0.00409

MarkerSeek score 49.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 5
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	874-876 bp	1.0	78.4
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	877-879 bp	1.0	77.7
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	906-908 bp	1.0	77.5
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	909-911 bp	1.0	76.8
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	55.0	50.0	57.9	57.6	879-881 bp	1.0	76.4