Hotspot

matK-trnK-UUU

LSC · 3569-4241 · 673 bp

Back to Raphanus

Pi 0.00297

MarkerSeek score 48.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 3
pi: 0.00297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GAGCCCCATCGAACTCTAAA	ACGCACACATGACTGGATTT	50.0	45.0	57.0	58.1	892 bp	1.0	71.7
matK-trnK-UUU_p2	GAGCCCCATCGAACTCTAAA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.0	58.2	803-804 bp	1.0	71.1
matK-trnK-UUU_p3	GAGCCCCATCGAACTCTAAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.0	58.2	802-803 bp	1.0	71.1
matK-trnK-UUU_p4	CGAGCCCCATCGAACTCTAA	ACGCACACATGACTGGATTT	55.0	45.0	59.3	58.1	893 bp	1.0	69.5
matK-trnK-UUU_p5	CGAGCCCCATCGAACTCTAA	ACGGTAGAGTACTCGGCTTT	55.0	50.0	59.3	58.2	803-804 bp	1.0	68.9