Hotspot

trnH-GUG-psbA

LSC · 77-312 · 236 bp

Back to Raphanus

Pi 0.0198

MarkerSeek score 52.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 7
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GACCTAGCTGCTGTTGAGTC	50.0	55.0	57.9	58.0	303 bp	1.0	80.7
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GACCTAGCTGCTGTTGAGTC	50.0	55.0	58.2	58.0	304 bp	1.0	80.5
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	309 bp	1.0	80.2
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	312 bp	1.0	80.1
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	310 bp	1.0	80.0