Hotspot

ycf1-trnN-GUU

IRa · 130588-132067 · 1480 bp

Back to Ranunculus

Pi 0.2

MarkerSeek score 72.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1480
variable_sites: 651
pi: 0.2
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GCCCAACTAACCAACCAAGA	TTGTGTTTTACTCCCCGCAA	50.0	45.0	58.0	57.9	2463-2647 bp	0.136364	45.2
ycf1-trnN-GUU_p2	GCCCAACTAACCAACCAAGA	TTTGTGTTTTACTCCCCGCA	50.0	45.0	58.0	57.9	2464-2648 bp	0.136364	45.2
ycf1-trnN-GUU_p3	GCCCAACTAACCAACCAAGA	GAACTTGGGTGTGGGTCTTT	50.0	50.0	58.0	57.9	2562-2746 bp	0.136364	45.1
ycf1-trnN-GUU_p4	GCCCAACTAACCAACCAAGA	TCAAAGCGAGCCCTTTCATT	50.0	45.0	58.0	58.1	2153-2337 bp	0.136364	45.0
ycf1-trnN-GUU_p5	GCCCAACTAACCAACCAAGA	TTACTCCCCGCAACTTTTCC	50.0	50.0	58.0	58.1	2456-2640 bp	0.136364	44.9