Hotspot

rpl32-trnL-UAG

SSC · 114889-115573 · 685 bp

Back to Ranunculus

Pi 0.0947

MarkerSeek score 75.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 190
pi: 0.0947
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAGAATGCGCGAATTAACCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1125-1229 bp	0.5	67.2
rpl32-trnL-UAG_p2	GAGAATGCGCGAATTAACCC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1126-1230 bp	0.5	67.2
rpl32-trnL-UAG_p3	GAGAATGCGCGAATTAACCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	1135-1239 bp	0.5	66.3
rpl32-trnL-UAG_p4	GAGAATGCGCGAATTAACCC	AAGCGCCGCATAACTCAATA	50.0	45.0	57.6	58.1	1737-1840 bp	0.454545	62.0
rpl32-trnL-UAG_p5	GAGAATGCGCGAATTAACCC	CCTTTTTCCGACGAATGGGA	50.0	50.0	57.6	58.2	1418-1521 bp	0.272727	55.6