Hotspot

ndhF-rpl32

SSC · 113841-114726 · 886 bp

Back to Ranunculus

Pi 0.0997

MarkerSeek score 78.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 886
variable_sites: 241
pi: 0.0997
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTCGGGAAAAGGAGAAGACC	AAGAGCCTTCGTTGTAACCG	55.0	50.0	57.9	58.2	1658-1728 bp	0.181818	52.9
ndhF-rpl32_p2	CAAGAGGTCGTGTGAACCAA	AAGAGCCTTCGTTGTAACCG	50.0	50.0	58.1	58.2	2077-2147 bp	0.181818	52.2
ndhF-rpl32_p3	GATTTAGCAACCGCACCAAC	AAGAGCCTTCGTTGTAACCG	50.0	50.0	58.0	58.2	2288-2358 bp	0.181818	52.0
ndhF-rpl32_p4	AGATTTAGCAACCGCACCAA	AAGAGCCTTCGTTGTAACCG	45.0	50.0	58.1	58.2	2289-2359 bp	0.181818	51.5
ndhF-rpl32_p5	GCAACCGCACCAACAAATAA	AAGAGCCTTCGTTGTAACCG	45.0	50.0	57.9	58.2	2282-2352 bp	0.181818	51.1