Hotspot

trnN-GUU-ycf1

IRb · 109871-111350 · 1480 bp

Back to Ranunculus

Pi 0.155

MarkerSeek score 69.30

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 1480
variable_sites: 533
pi: 0.155
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	TTGTGTTTTACTCCCCGCAA	AGAGGACTAGCCGACCTTAC	45.0	55.0	57.9	57.9	2327-2564 bp	0.181818	57.3
trnN-GUU-ycf1_p2	TTGTGTTTTACTCCCCGCAA	GAGGACTAGCCGACCTTACT	45.0	55.0	57.9	57.9	2326-2563 bp	0.181818	57.3
trnN-GUU-ycf1_p3	TTTGTGTTTTACTCCCCGCA	AGAGGACTAGCCGACCTTAC	45.0	55.0	57.9	57.9	2328-2565 bp	0.181818	57.3
trnN-GUU-ycf1_p4	TTTGTGTTTTACTCCCCGCA	GAGGACTAGCCGACCTTACT	45.0	55.0	57.9	57.9	2327-2564 bp	0.181818	57.3
trnN-GUU-ycf1_p5	GAACTTGGGTGTGGGTCTTT	GAGGACTAGCCGACCTTACT	50.0	55.0	57.9	57.9	2425-2662 bp	0.181818	57.0