Hotspot

psbE-petL

LSC · 65402-66552 · 1151 bp

Back to Ranunculus

Pi 0.046

MarkerSeek score 83.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 159
pi: 0.046
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	TTAAAGCCGCCAGGAGAAAA	50.0	45.0	57.9	57.7	1278-1360 bp	1.0	89.3
psbE-petL_p2	ACTGACGAATAACCAACCCG	TTAAAGCCGCCAGGAGAAAA	50.0	45.0	57.9	57.7	1291-1373 bp	1.0	89.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	TTAAAGCCGCCAGGAGAAAA	45.0	45.0	58.2	57.7	1287-1369 bp	1.0	88.4
psbE-petL_p4	CGAATAACCAACCCGCAATG	TTAAAGCCGCCAGGAGAAAA	50.0	45.0	57.8	57.7	1286-1368 bp	1.0	88.3
psbE-petL_p5	CAACCCGCAATGAATAGGGA	TAGTTAAAGCCGCCAGGAGA	50.0	50.0	57.9	58.4	1281-1363 bp	1.0	88.1