Hotspot

psaA-ycf3

LSC · 42447-43212 · 766 bp

Back to Ranunculus

Pi 0.0502

MarkerSeek score 77.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 121
pi: 0.0502
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TAATGGAACCAACCGGCAAA	TATACCGATAGCAGGGGGTG	45.0	55.0	58.0	58.1	1648-1735 bp	1.0	91.4
psaA-ycf3_p2	TAATGGAACCAACCGGCAAA	TGGTTGAAGATTACGAGGCG	45.0	50.0	58.0	58.0	1271-1329 bp	0.772727	82.6
psaA-ycf3_p3	TAATGGAACCAACCGGCAAA	GTTGAAGATTACGAGGCGGT	45.0	50.0	58.0	58.0	1269-1327 bp	0.772727	82.6
psaA-ycf3_p4	ACTTTGGAGCAGCTTTGTGA	TGGTTGAAGATTACGAGGCG	45.0	50.0	57.9	58.0	1291-1349 bp	0.772727	82.0
psaA-ycf3_p5	ACTTTGGAGCAGCTTTGTGA	GTTGAAGATTACGAGGCGGT	45.0	50.0	57.9	58.0	1289-1347 bp	0.772727	82.0