Hotspot

trnK-UUU-rps16

LSC · 4226-4709 · 484 bp

Back to Ranunculus

Pi 0.0629

MarkerSeek score 75.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 80
pi: 0.0629
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CACATTCGTCTCCACTCAGG	ACGGGAATCAAGCATTCACA	55.0	45.0	58.0	57.8	1682-1775 bp	0.909091	86.8
trnK-UUU-rps16_p2	CACATTCGTCTCCACTCAGG	CTTGAAAAAGGCGCTCAACC	55.0	50.0	58.0	58.2	1040-1124 bp	0.909091	86.5
trnK-UUU-rps16_p3	CACATTCGTCTCCACTCAGG	ATTCTTTCTCGAGCCGTACG	55.0	50.0	58.0	57.8	1241-1325 bp	0.863636	85.0
trnK-UUU-rps16_p4	CACATTCGTCTCCACTCAGG	ACACGGGAATCAAGCATTCA	55.0	45.0	58.0	57.8	1684-1777 bp	0.636364	75.4
trnK-UUU-rps16_p5	CACATTCGTCTCCACTCAGG	TCACACGGGAATCAAGCATT	55.0	45.0	58.0	57.8	1686-1779 bp	0.636364	75.4