Hotspot

psbE-petL

LSC · 107756-109285 · 1530 bp

Back to Racinaea

Pi 0.00829

MarkerSeek score 60.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1530
variable_sites: 19
pi: 0.00829
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1654-1677 bp	1.0	76.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1653-1676 bp	1.0	76.9
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1667-1690 bp	1.0	76.6
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1666-1689 bp	1.0	76.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1662-1685 bp	1.0	76.0