Hotspot

petA-psbJ

LSC · 105854-106986 · 1133 bp

Back to Racinaea

Pi 0.0118

MarkerSeek score 56.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 20
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	1247-1259 bp	1.0	81.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	1245-1257 bp	1.0	81.3
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	1246-1258 bp	1.0	78.3
petA-psbJ_p4	GCCTTTTGTTCTTCTTCGCA	TCTGGGTTGGGTTCATCTCT	45.0	50.0	57.2	58.0	1246-1258 bp	1.0	75.5
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TGGGTTGGGTTCATCTCTCT	45.0	50.0	57.2	58.0	1244-1256 bp	1.0	75.5