Hotspot

rpoB-trnC-GCA

LSC · 68489-69823 · 1335 bp

Back to Racinaea

Pi 0.009

MarkerSeek score 64.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1335
variable_sites: 18
pi: 0.009
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1402-1429 bp	1.0	77.5
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	1403-1430 bp	1.0	77.2
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1428-1455 bp	1.0	74.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1429-1456 bp	1.0	74.0
rpoB-trnC-GCA_p5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.1	58.1	1479-1506 bp	1.0	73.8